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Abstract Plastic litter is a globally pervasive pollutant. Storms are likely key drivers of plastic transport to oceans, but plastic transport during rising and falling limbs of storm hydrographs is rarely measured. Measurements of plastic movement throughout individual storms will improve watershed models of plastic dynamics. We used cameras to quantify macroplastic movement (i.e., particles > 5 mm) in rivers before, during, and after individual storms (N = 18) at 10 sites within three North American watersheds. Most storms showed no difference in macroplastic transport between rising and falling hydrograph limbs or evidence of hysteresis (transport rate range = 0–236 items/30 min). Total macroplastic exported during storm events was positively related to storm magnitude and was greatest at more urban sites. Thus, macroplastic transport during storms was driven by storm size and land use. The quantitative relationships between macroplastic movement and hydrology will improve discharge‐weighted calculations of macroplastic transport which can benefit modeling, monitoring, and mitigation efforts. Practitioner PointsMacroplastic particles (i.e, > 5 mm) are both retained in urban streams (e.g., in debris dams), and move downstream during baseflow and stormflow conditionsStorm flows are key periods of macroplastic transport: transport rates are higher on both rising and falling limbs of storm hydrographs relative to baseflow.The amount of macroplastics moving during storm flows is positively related to storm intensity.The predictive relationships generated between storm flow and macroplastic transport will improve estimates of annual export, and policies for macroplastic pollution reduction. 

Plastic pollution is a defining environmental contaminant and is considered to be one of the greatest environmental threats of the Anthropocene, with its presence documented across aquatic and terrestrial ecosystems. The majority of this plastic debris falls into the micro (1 μm–5 mm) or nano (1–1000 nm) size range and comes from primary and secondary sources. Its small size makes it cumbersome to isolate and analyze reproducibly, and its ubiquitous distribution creates numerous challenges when controlling for background contamination across matrices (e.g., sediment, tissue, water, air). Although research on microplastics represents a relatively nascent subfield, burgeoning interest in questions surrounding the fate and effects of these debris items creates a pressing need for harmonized sampling protocols and quality control approaches. For results across laboratories to be reproducible and comparable, it is imperative that guidelines based on vetted protocols be readily available to research groups, many of which are either new to plastics research or, as with any new subfield, have arrived at current approaches through a process of trial-and-error rather than in consultation with the greater scientific community. The goals of this manuscript are to (i) outline the steps necessary to conduct general as well as matrix-specific quality assurance and quality control based on sample type and associated constraints, (ii) briefly review current findings across matrices, and (iii) provide guidance for the design of sampling regimes. Specific attention is paid to the source of microplastic pollution as well as the pathway by which contamination occurs, with details provided regarding each step in the process from generating appropriate questions to sampling design and collection.